Free Fatty Acids (FFA)

General Information

The measurement of 3-hydoxy-butyrate is used together with free fatty acids (FFA) as a useful first line indicator of inherited defects of mitochondrial fatty acid catabolism. Ideally specimens should be taken during acute hypoglycaemic episodes or after a provocative fast.

In most circumstances, as blood glucose falls, lipolysis and subsequently ketogenesis are stimulated; thus the plasma concentration of FFA and 3-hydroxybutyrate increase in an approximately equimolar fashion. In fatty acid oxidation defects, lipolysis will still be stimulated in response to a drop in blood glucose, but ketogenesis will be reduced. Therefore, a disproportionately high free fatty acid concentration relative to a low 3-hydroxy-butyrate concentration is found, particularly during periods of stress i.e. infection, coma, fasting and hypoglycaemia. In patients with hyperinsulinism, FFA and 3-hydroxybutyrate levels will both be low, as insulin suppresses lipolysis. This pattern will also be observed in patients with hypopituitarism, therefore this test can not be used to diagnose hyperinsulinism. However, hyperinsulinism can be ruled out by the demonstration of ketosis, i.e. elevated FFA and 3-hydroxy butyrate in the presence of hypoglycaemia.

Seehttp://www.metbio.net/metbioHome.asp for Best Practice Guidelines for the Investigation of Hypoglycaemia in Infants and Children.