Specimen TransportSamples should arrive in laboratory on same day. No Friday service available.
Sample Processing in LaboratoryLithium Heparin blood (5mls) may also be used, and transferred under aseptic conditions in the immunology Laboratory (by prior arrangement).
Turnaround Time7 Days
Quantiferon Plus Interferon gamma release assay for TB
Quantiferon Plus IGRA test can be used to detect exposure to Mycobacterium Tuberculosis.
Special Collection Arrangements apply see section below on blood collection. This assay is currently only available within Heart of England NHS trust sites and by prior arrangement with the laboratory. Please contact the laboratory on 42184 to arrange. This assay is currently not available through Primary Care
The QuantiFERON-TB Gold Plus IT system uses four dedicated blood collection vacutainers containing specific M. tuberculosis proteins or controls (NIL, TB1,TB2, MITO tubes). Tubes are cultured overnight, and the amount of IFN-γproduced is measured by enzyme linked immunoabsorption assay (ELISA). Results for test samples are reported in International Units (IU) relative to a standard curve prepared by testing dilutions of manuracturer supplied IFN-γ.
QuantiFERON TB Gold Plus IT is recommended for routine screening of all patients with normal lymphocyte counts, TB contacts, and healthcare worker pre-employment screening.
Blood Collection instructions
RESULTS - Positive, Negative, Borderline and Indeterminate results.
A negative result suggests no active immune response to Mycobacterium Tuberculosis. A positive results indicates a response to MTB infection, but does not distinguish between active and latent disease. The test cannot be used to exclude active infection, since false-negative results do occur. It should always be interpreted in the context of other clinial and laboratory features.
Due to biological variations, re-testing borderline results is recommended. Indeterminate results indicate failure of positive or negative controls which may be due to technical or patient-specific factors. Re-testing recommended.
- EQA Scheme?: Yes
- EQA Status: Full EQA scheme