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Department

Microbiology

Preferred Sample Type

Hepatitis C Genotyping

Suitable Specimen Types

  • EDTA Whole Blood
At least 2ml plasma. Please provide patients HCV viral load on the request form. HCV viral load must be >200 IU/ml for the assay to be able to detect a genotype.

Turnaround Time

14 days

Sample Stability

Freshly drawn specimens (whole blood) may be held at 2 to 30°C for up to 6 hours prior to centrifugation. Plasma samples are stored for 2 years at -20°C

Hepatitis C Genotyping

General Information

Based on genetic similarity, HCV has been classified into six major genotypes (1-6) and numerous subtypes. HCV genotype is predictive of the response of HCV-infected patients to peg-interferon/ribavirin combination therapy. Before starting combination therapy, it is recommended that the genotype be determined so that the patient can receive the most appropriate therapy regimen. The Abbott RealTime HCV Genotype II is an in vitro reverse transcription-polymerase chain reaction (RT-PCR) assay for determining the genotype(s) of hepatitis C virus (HCV) in plasma and serum from HCV-infected individuals.

Patient Preparation

For taking blood samples we recommend that users follow either their local protocols or “The Royal Marsden Manual of Clinical Nursing Procedures”.

Lavender  top blood (EDTA) sample

  • Sufficient blood for the test should be drawn into lavender topped (EDTA) vacuum tubes
  • Invert tube 8–10 times.

Specifications

  • EQA Scheme?: Yes
  • EQA Status: QCMD & NEQAS