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Department

Microbiology

Preferred Sample Type

Hepatitis B viral load

Suitable Specimen Types

  • Serum
2ml serum

Turnaround Time

5 days

Hepatitis B viral load

General Information

The Alinity m HBV assay is an in vitro polymerase chain reaction (PCR) assay for the quantitation of Hepatitis B Virus (HBV) DNA from HBV-infected individuals over the range of 30 to 1,000,000,000 IU/ml. The assay targets a region on the surface antigen gene which gives the detection of HBV genotypes A, B, C, D, E, F, G, H and I.

Patient Preparation

For taking blood samples we recommend that users follow either their local protocols or “The Royal Marsden Manual of Clinical Nursing Procedures”.

  • Sufficient blood for the test should be drawn into an EDTA vacuum tube
  • Invert tube 8–10 times.

 

Healthcare Workers

Samples from healthcare workers for exposure prone procedure clearance should be clearly labelled.

Please use the appropriate form and complete all relevant details. Form available here.

Refer to UKAP Guidelines: Integrated Guidance on Health Clearance of Healthcare Workers and the Management of Healthcare Workers living with bloodborne viruses. November 2023.

Notes

Sample Stability

Please transport samples to the laboratory for testing ASAP. Primary tubes can be tested up to 24hrs after drawing blood as long as the tubes have been kept at +4◦C. Separated plasma samples can be kept at +4◦C for 5 days before testing.

Debris within serum specimens (e.g. clots, fibrin strands) may interfere with sample processing as a result of insufficient mixing on draw.

Specifications

  • EQA Scheme?: Yes
  • EQA Status: QCMD and NEQAS