Preferred Sample Type
Suitable Specimen Types
- Serum
- Li Hep Plasma
Sample Processing in Laboratory
Usual
Sample Preparation
Centrifuge
Turnaround Time
1 hour (urgent pathway)Sample Stability
On-gel stability: 24 hours Room Temperature, 72 hours 2-8 degrees Celsius, store at -20 degrees Celsius for longer storage.Troponin I - High Sensitive
General Information
Troponin I is a part of the troponin complex. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle. The letter I is given due to its inhibitory character.
Patient Preparation
None
Notes
K -EDTA, Lithium heparin and sodium citrate plasma. The Cardiology Nurse Practitioners have elected to continue using lithium heparin tubes, to ensure a faster turnround time .
Reference Range
The cut-off values used for the Abbott Alinity Troponin I assay are based on 99th percentiles and are as below:
- Male <34 ng/L
- Female <16 ng/L
Specifications
- EQA Scheme?: Yes
-
EQA Status:
UK NEQAS cardiac markers