Preferred Sample Type

16S PCR and Sequencing

Suitable Specimen Types

  • Culture Slope
  • Sterile Site Sample
Min volume for sterile site
samples is 600ul or pea size

Turnaround Time

7 days

Sample Stability

Samples should be kept at 4°C and sent to the laboratory
as soon as possible for testing.

16S PCR and Sequencing

General Information

16S PCR and Sequencing can be used for identification of bacterial isolates or for the detection and identification of bacterial DNA in sterile site samples.

Sterile site samples should always have conventional M,C+S testing as well as 16S.

Patient Preparation

For general aspects of taking tissue samples we recommend that users follow either their local protocols or “The Royal Marsden Manual of Clinical Nursing Procedures”. 

Tissue samples for microbiology

  • All tissue samples should be collected aseptically and transferred directly to sterile sample
    containers with no additives of any kind.

Fluid, Aspirate and Pus Samples

  • All fluid and pus samples should be collected aseptically and transferred directly to sterile sample containers with no additives of any kind.



Preferred sample types:

Bacterial isolates for identification on slope culture


Any sterile site sample in a sterile plastic container. Sterile site samples must not be diluted with or suspended in any carrier fluid.

Samples should be submitted on the correct request form and clinical details should be included. All results receive technical and clinical validation.

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HTA licence number is 12366

Protection of Personal Information – Laboratory Medicine comply with the Trust Data Protection Policy and have procedures in place to allow the Directorate and it’s employees to comply with the Data Protection Act 1998 and associated best practice and guidance.

The Trust Laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital were awarded UKAS (United Kingdom Accreditation Service) accreditation to the internationally recognised ISO 15189 standard in May 2015. For a list of accredited tests and other information please visit the test database
Tests not appearing on this scope are either under consideration or in the process of accreditation and so currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235