Cystic Fibrosis is the most common serious genetic condition in Caucasians, with an incidence of approximately 1 in 2500 live births in the UK. It is a generalised disorder of excessive viscous exocrine secretions, due to impaired chloride transport across membranes caused by mutations in the CFTR gene. The main symptoms at presentation are recurrent chest and lung infections, cough and wheeze, failure to thrive, pancreatic insufficiency leading to malabsorption and male infertility.
Sweat chloride and conductivity/sodium concentrations are increased in cystic fibrosis, and their measurement provides the definitive test for diagnosis of cystic fibrosis.
Localised sweating is induced on the flexor surface of an arm by Pilocarpine iontophoresis (introduction if ions of soluble salts into the body by an electric current) using Pilocarpine gels. Sweat is collected by the Wescor Macroduct system, which collects sweat passively and automatically, driven by the same hydraulic pressure that causes sweat to move from the base of the sweat gland to the skin surface. After removing the Macroduct sweat collector, the sweat sodium and chloride are measured directly by ICP-MS using neat sweat.
From 08/11/2016 sweat analysis is performed by ICP-MS. There is no change to the interpretation of results.
Sweat tests are booked via the Clinical Chemistry office, Laboratory Medicine, Birmingham Heartlands Hospital.
There is one sweat test clinic per week - either adult or paediatric depending on demand. Adult clinics are held on Monday mornings, and paediatric clinics are held on Monday afternoons.
Sweat sodium is used as a check of the chloride result. Interpretation is as follows:
Cystic fibrosis unlikely < 50 mmol/L
Supports the diagnosis of CF > 90 mmol/L
The Sodium and Chloride concentrations should be within 20 mmol/L of each other.
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The Trust Laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital were awarded UKAS (United Kingdom Accreditation Service) accreditation to the internationally recognised ISO 15189 standard in May 2015. For a list of accredited tests and other information please visit the test database http://www.heftpathology.com/frontpage/test-database.html.
Tests not appearing on this scope are either under consideration or in the process of accreditation and so currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.
For further information contact Louise Fallon, Quality Manager, 0121 424 1235