Department

Microbiology

Preferred Sample Type

Respiratory Viruses PCR

Suitable Specimen Types

  • Sputum
  • BAL
  • Trap
  • NPA
  • BAL
  • Trap
  • Nose and Throat Swab
  • Throat Swab
1ml

Turnaround Time

3 days

Sample Stability

Stored for 6 months

Respiratory Viruses PCR

General Information

The following tests are included for all patients Respiratory Syncytial Virus (RSV), Influenza A, Influenza B, Parainfluenza virus, Rhinovirus, Human Metapneumovirus, Adenovirus and Swine Flu. Immunocompromised patients, Neonates and patients on ITU/HDU will have the following additional tests Herpes simplex (HSV), Cytomegalovirus (CMV) and Varicella zoster virus (VZV)

Patient Preparation

For general aspects of taking respiratory samples we recommend that users follow either their local protocols or “The Royal Marsden Manual of Clinical Nursing Procedures”.

 Sputum

    Ask patient to take three deep breaths in through their nose, exhale through pursed lips and then force a deep cough. Ask patient to expectorate into a 20ml white top sterile sample container.

Bronchoalveolar Lavage (BAL)

    BAL should be collected according to local protocols using sterile fluids as the instillate. Recovered lavage fluid should be aseptically transferred to a 20ml white top sterile sample container

Nasopharyngeal Aspirate (NPA)

    NPA should be collected according to local protocols using sterile fluids as the instillate. Recovered mucus/fluid should sent in a 20ml white top sterile sample container or other well sealed sterile container.

Nasopharyngeal wash: vacuum-assisted (trap) aspirate method

    Nasopharyngeal wash should be collected according to local protocols using sterile fluids as the instillate. Recovered mucus/fluid should sent in a 20ml white top sterile sample container or other well sealed sterile container.

Nose and throat swabs for virology / molecular tests

    Use a green topped virology transport medium kit (labelled as Green Top Sigma-VIROCULT swab).

    Throat: Ask patient to sit upright facing a strong light, tilt head backwards, open mouth and stick out tongue. Depress tongue with a spatula. Ask patient to say ‘Ah’. Quickly but gently roll the swab over the tonsils and posterior pharynx

    Nose: Ask patient to tilt head backwards. Insert swab inside the anterior nares with the tip directed upwards and gently rotate.

    Place the swab(s) in the transport liquid and snap off the swab shaft at the score line so it fits into the sample pot. Close the cap tightly and label with patient information. Label as “nose”, “throat” or “nose and throat”

Notes

Please indicate if patient is immunocompromised. Significant results telephoned. If Avian Flu is suspected contact Duty Virologist immediately. Please note that we are able to test for Oseltamivir resistance only on request of the Consultant Virologist

Specifications

  • EQA Scheme?: Yes
  • EQA Status: QCMD & H5 Influenza EQA

Copyright heftpathology 2013, 2014, 2015, 2016, 2017, 2018

HTA licence number is 12366

Protection of Personal Information – Laboratory Medicine comply with the Trust Data Protection Policy and have procedures in place to allow the Directorate and it’s employees to comply with the Data Protection Act 1998 and associated best practice and guidance.

The Trust Laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital were awarded UKAS (United Kingdom Accreditation Service) accreditation to the internationally recognised ISO 15189 standard in May 2015. For a list of accredited tests and other information please visit the test database http://www.heftpathology.com/frontpage/test-database.html.
Tests not appearing on this scope are either under consideration or in the process of accreditation and so currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235

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