Thiamine pyrophosphate is coenzyme to three systems one of which is transketolase: situated within the hexose monophosphate shunt. It mediates transfer of CH2OHC=O between donor and acceptor sugars. This enzyme is important in erythrocyte metabolism and its activity can therefore be used as an index of thiamine nutrition.
Insufficient dietary thiamine leads to partial inactivation of all three enzyme systems which affects the CNS before other tissues and a spectrum of neurological disorders can result including peripheral neuropathy, Wernicke’s encephalopathy and Korsakof’s psychosis.
The transketolase enzyme can be saturated by thiamine (vitamin B1) within 24 hours of vitamin therapy or return to an adequate diet. Therefore, it is desirable for the patient’s blood to be sampled as soon after admission as possible and prior to any intake of food.
Inform duty biochemist (Bleep 2506) when test is to be performed. (N.B. must get it to Medical School same day).
Ensure patient has not had any vitamin supplements since this saturates the red cells with thiamine making the test useless.
Collect sample and send to lab immediately otherwise cool sample ON ICE.
Reference lab will provide information.
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The Trust Laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital were awarded UKAS (United Kingdom Accreditation Service) accreditation to the internationally recognised ISO 15189 standard in May 2015. For a list of accredited tests and other information please visit the test database http://www.heftpathology.com/frontpage/test-database.html.
Tests not appearing on this scope are either under consideration or in the process of accreditation and so currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.
For further information contact Louise Fallon, Quality Manager, 0121 424 1235