Preferred Sample Type

Non-HDL Cholesterol (calculated)

Suitable Specimen Types

  • Serum
  • EDTA Plasma
  • Li Hep Plasma
5-10ml whole blood

Sample Processing in Laboratory


Sample Preparation

Centrifuge specimens

Turnaround Time

1 day

Sample Stability


Non-HDL Cholesterol (calculated)

General Information

Non-HDL cholesterol is a calculated parameter derived by subtracting HDL cholesterol from total cholesterol. Non HDL cholesterol provides an estimation of all cholesterol containing (atherogenic) particles (LDL, triglyceride-rich very low density lipoprotein and so-called remnant particles), thus providing a more accurate assessment of CVD risk compared to LDL alone.

Non-HDL cholesterol levels are requested as part of a lipid profile, along with total cholesterol, HDL, LDL and triglycerides. 

Non-HDL cholesterol concentrations >7.5mmol/L warrant specialist lipid clinic referral.

If high intensity statins are used, then non-HDL-cholesterol should be lowered by at least 40% from baseline (NICE 2014).



Patient Preparation

Non-fasting specimens are accepted


Non HDL cholesterol is calculated by subtracting HDL from Total Cholesterol (TC).

Non-HDL cholesterol = TC - HDL

This calculation should not be used when plasma triglyceride concentration exceeds 10.0 mmol/L


  • EQA Scheme?: No

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HTA licence number is 12366

Protection of Personal Information – Laboratory Medicine comply with the Trust Data Protection Policy and have procedures in place to allow the Directorate and it’s employees to comply with the Data Protection Act 1998 and associated best practice and guidance.

The Trust Laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital were awarded UKAS (United Kingdom Accreditation Service) accreditation to the internationally recognised ISO 15189 standard in May 2015. For a list of accredited tests and other information please visit the test database
Tests not appearing on this scope are either under consideration or in the process of accreditation and so currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235

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