Preferred Sample Type

FMH - Feto Maternal Haemorrhage

Suitable Specimen Types

  • EDTA Whole Blood

Specimen Transport

Standard transport to laboratory

Sample Preparation


Turnaround Time

24 hrs

Sample Stability

4°C for 3 days

FMH - Feto Maternal Haemorrhage

General Information

BCSH guidelines (2009)

Guidelines state that :

“Flow cytometry tests for a minor D positive population may be required:

  • Following a D positive RBC transfusion to a D negative woman of childbearing potential. To estimate or confirm the dose of anti-D immunoglobulin required, as part of the protocol given in the anti-D guidelines (BCSH 2006a), to prevent sensitisation to the D antigen.
  • In solid organ transplantation when the donor is D positive and recipient D negative with childbearing potential.”


“The sample should be referred for quantification of D positive cells by FC if the FMH is greater than 2 mL by the Acid Elution method”.

FMH by flow utilises the an FITC conjugated monoclonal  anti-D reagent.

Samples within HEFT will automatically be refered for FMH estimation by flow cytometry if found to be high by the Kleihauer method.

Suggested Anti-D dosage takes into account the uncertainty of measurement of FMH measured by flow cytometry in this laboratory. Click here for further information.

Download the FMH request form here

Link to immunophenotyping pages



Please indicate any transfusion history or recent anti-D immunoglobulin


  • EQA Scheme?: Yes
  • EQA Status: NEQAS

The laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital form part of the services provided by University Hospitals Birmingham and are UKAS (United Kingdom Accreditation Service) accredited to the ISO 15189:2012 standard. For a list of accredited tests and other information please visit the UKAS website using the following link:

  • Heartlands, Good Hope and Solihull Hospital pathology laboratories are a UKAS accredited medical laboratory No.8217
  • United Kingdom Health Security Agency laboratory is a UKAS accredited medical laboratory No.8213

Tests not appearing on the UKAS Schedule of Accreditation currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further test information, please visit the test database:

Protection of personal information - Laboratory Medicine comply with the Trust Data Protection policy and have procedures in place to allow the Directorate and its employees to comply with the Data Protection act  1998 and associated best practice and guidance.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235