Preferred Sample Type

EUROclass B cell extended immunophenotyping

Suitable Specimen Types

  • Li Hep
  • EDTA Whole Blood
1 ml

Specimen Transport

No special requirements

Sample Processing in Laboratory

Sample should be stored between at room temperature (18C and 26C) prior to staining.

Turnaround Time

Up to 28 days

Sample Stability

Ideally EDTA sample should be <48 hours old and Li Hep <24 hrs old

EUROclass B cell extended immunophenotyping

General Information

Extended B cell immunophenotyping is used for assessment of immunodeficiency and immune competence in patients where B cell function (humoral immunity) is compromised.  It is currently used as a research tool, as it has not been diagnostically validated, but is informative in primary antibody immunodeficiency conditions such as common variable immunodeficiency (CVID), hyper-IgM syndromes (CD40L, CD40, AID, UNG and NEMO), and in XLP (SAP and XIAP); assessing B cell subset reconstitution after stem cell or bone marrow transplant and assessing response to B cell-depleting immunotherapy (eg Rituximab).  It may be informative in identifying defects in TACI and BAFF-R in patients presenting with clinical symptoms and other laboratory features consistent with CVID.  Notably, transitional cells are also increased in SLE and Sjogrens syndrome, and plasmablasts are increased in multiple myeloma.  Irreversible depletion of memory B cells is also described in HIV-1 infection.


Patient Preparation

No Specific preparation required

Reference Range

  EUROclass classification (from Wehr et al 2008).


>1% B cells


< 1% CD19+ B cells


>2% switched memory B cells



<2% switched memory B cells

21 low         

>10% CD21 low B cells


21 norm  

<10% CD21 low B cells


Tr hi      

>9% transitional B cells


Tr norm   

<9% transitional B cells





  • EQA Scheme?: No
  • EQA Status: This is a research assay only and not available for routine use.

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The Trust Laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital were awarded UKAS (United Kingdom Accreditation Service) accreditation to the internationally recognised ISO 15189 standard in May 2015. For a list of accredited tests and other information please visit the test database
Tests not appearing on this scope are either under consideration or in the process of accreditation and so currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235

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