Local code : ENAS

Preferred Sample Type

ENA antibody screen

Suitable Specimen Types

  • Serum
5-10 mL of serum

Sample Processing in Laboratory

Stored in laboratory at 4 C

Turnaround Time

5 days

Sample Stability

Stored in laboratory at 4 C

ENA antibody screen

General Information

The detection of antinuclear antibodies (ANAs) has long been an important tool in the diagnosis of systemic rheumatic diseases. The antigens used in their detection are purified by the saline extraction of human or animal nuclei, this has led to them being called extractable nuclear antigens (ENA). The most commonly measured ENA specifications are anti-SS-A/Ro, anti-SS-B/La, anti-Sm D, anti-RNP anti-Jo-1 and anti-SCL-70. Individual ENA types are performed if the ENA screen is positive.

Patient Preparation

None required

Reference Range

A normal result would be Negative


  • EQA Scheme?: Yes

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HTA licence number is 12366

Protection of Personal Information – Laboratory Medicine comply with the Trust Data Protection Policy and have procedures in place to allow the Directorate and it’s employees to comply with the Data Protection Act 1998 and associated best practice and guidance.

The Trust Laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital were awarded UKAS (United Kingdom Accreditation Service) accreditation to the internationally recognised ISO 15189 standard in May 2015. For a list of accredited tests and other information please visit the test database
Tests not appearing on this scope are either under consideration or in the process of accreditation and so currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235

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