Local code : CRYO

Preferred Sample Type


Suitable Specimen Types

  • Serum
  • EDTA Whole Blood
5 mL clotted blood ( non anti-coagulated) AND 5ml EDTA whole blood.

Specimen Transport

Transported to laboratory in portable incubator at 37 C. For more information contact Immunology at Heartlands contact 42105. Good Hope Hospital contact Biochemistry 47250 or 49249, Queen Elizabeth contact Biochemistry Specimen reception 15985

Sample Processing in Laboratory

Incubator at 37 C

Sample Preparation

This assay requires both serum AND EDTA plasma samples. Samples must be taken into pre-warmed vacutainers.

Samples should be transported to laboratory in portable incubator at 37 C

Turnaround Time

10 days


General Information

Test should be requested in patients with cold-induced skin rashes and some patients with renal and joint diseases. A low complement C4 level with or without a rheumatoid factor should also suggest a possible cryoglobulin.

Clinically significant cryoglobulins are lost if the sample is allowed to cool below body temperature even for a short period. Once the sample has clotted and is separated it may be handled as any other sample.

Patient Preparation




Cryoprotein Phlebotomy Instructions

Specimens requiring cryoprotein investigation MUST be taken and maintained at 37ºC until clotted and separated in the laboratory.

The samples must be returned to the laboratory within 2 hours to ensure the temperature is maintained above 37ºC.

Use of the incubator:

  1. Remove the pre-warmed phlebotomy equipment and blood tubes from the incubator.
  2. Place the heat packs and clear plastic lid back in place as shown below to prevent heat loss from the incubator.



  1. A minimum of 2 plain red top tubes (with no gel) AND 1 EDTA tube must be taken.
  2. Samples must be taken immediately and returned to the incubator, inside the heat packs.
  3. Seal the incubator lid and return to the laboratory immediately.

Please contact the (BHH) immunology team on ext. 42105 (0121 4242105) with any problems.

Good Hope Hospital contact Biochemistry 47250 or 49249

Queen Elizabeth contact Biochemistry specimen reception 15985

Reference Range



  • EQA Scheme?: Yes
  • EQA Status: UKNEQAS Pilot Scheme in place

The laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital form part of the services provided by University Hospitals Birmingham and are UKAS (United Kingdom Accreditation Service) accredited to the ISO 15189:2012 standard. For a list of accredited tests and other information please visit the UKAS website using the following link:

  • Heartlands, Good Hope and Solihull Hospital pathology laboratories are a UKAS accredited medical laboratory No.8217
  • United Kingdom Health Security Agency laboratory is a UKAS accredited medical laboratory No.8213

Tests not appearing on the UKAS Schedule of Accreditation currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further test information, please visit the test database:

Protection of personal information - Laboratory Medicine comply with the Trust Data Protection policy and have procedures in place to allow the Directorate and its employees to comply with the Data Protection act  1998 and associated best practice and guidance.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235