Preferred Sample Type

Alkaline Phosphatase (ALP)

Suitable Specimen Types

  • Serum
  • Li Hep Plasma
200 uL blood (1 x paed Lith Hep tube)

Specimen Transport

Not applicable

Sample Processing in Laboratory

No special requirements

Sample Preparation

Centrifuge on arrival

Turnaround Time

1 day

Sample Stability


Alkaline Phosphatase (ALP)

General Information

ALP activity changes markedly throughout childhood and rises rapidly during the adolescent growth spurt, particularly in boys. Increases in bone ALP of 3.4-4.0 times the adult range can be seen during this time. Values of over 1000 U/L in teenage boys or 800 U/L in teenage girls require investigation.

Patient Preparation



No significant interference from haemolysis, icterus or lipaemia.

EDTA contamination decreases ALP activity.

Anticonvulsants, barbiturates, oral contraceptives can increase ALP activity.

Reference Range

Neonate (<4weeks)   70-380 U/L

4wks-16yrs               60-425 U/L

Adult                        30-130 U/L

(Source : Pathology Harmony Recommendations)


  • EQA Scheme?: Yes
  • EQA Status:

    UK NEQAS for Clinical Chemistry

The laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital form part of the services provided by University Hospitals Birmingham and are UKAS (United Kingdom Accreditation Service) accredited to the ISO 15189:2012 standard. For a list of accredited tests and other information please visit the UKAS website using the following link:

  • Heartlands, Good Hope and Solihull Hospital pathology laboratories are a UKAS accredited medical laboratory No.8217
  • United Kingdom Health Security Agency laboratory is a UKAS accredited medical laboratory No.8213

Tests not appearing on the UKAS Schedule of Accreditation currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further test information, please visit the test database:

Protection of personal information - Laboratory Medicine comply with the Trust Data Protection policy and have procedures in place to allow the Directorate and its employees to comply with the Data Protection act  1998 and associated best practice and guidance.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235