Department

Microbiology

Preferred Sample Type

Adenovirus viral load

Suitable Specimen Types

  • EDTA Whole Blood
5ml EDTA

Turnaround Time

5 days

Sample Stability

Stored for 2 years

Adenovirus viral load

General Information

The assay was developed and published by the German National Reference Laboratory for Adenoviruses, Hannover Medical School, Germany.   Primer and probe sequences were designed against multiple reference sequences to ensure the detection of all 51 known human adenovirus types.   The test uses a single round real-time PCR targeting a highly conserved 133 bp region of the viral hexon gene. Specific detection and quantification is by real time Taqman hydrolysis probe analysis of the PCR amplicon, using the Applied Biosystems 7500 fast system.

Patient Preparation

For taking blood samples we recommend that users follow either their local protocols or “The Royal Marsden Manual of Clinical Nursing Procedures”.

Lavender  top blood (EDTA) sample

  • Sufficient blood for the test should be drawn into lavender topped (EDTA) vacuum tubes
  • Invert tube 8–10 times.

Notes

Significant Results Telephoned

Reference Range

Lower limit of detection 400copies per ml

Specifications

  • EQA Scheme?: Yes
  • EQA Status: QCMD

The laboratories at Heartlands Hospital, Good Hope Hospital and Solihull Hospital form part of the services provided by University Hospitals Birmingham and are UKAS (United Kingdom Accreditation Service) accredited to the ISO 15189:2012 standard. For a list of accredited tests and other information please visit the UKAS website using the following link: https://www.ukas.com/find-an-organisation/

  • Heartlands, Good Hope and Solihull Hospital pathology laboratories are a UKAS accredited medical laboratory No.8217
  • United Kingdom Health Security Agency laboratory is a UKAS accredited medical laboratory No.8213

Tests not appearing on the UKAS Schedule of Accreditation currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further test information, please visit the test database: http://www.heftpathology.com/frontpage/test-database.html.

Protection of personal information - Laboratory Medicine comply with the Trust Data Protection policy and have procedures in place to allow the Directorate and its employees to comply with the Data Protection act  1998 and associated best practice and guidance.

For further information contact Louise Fallon, Quality Manager, 0121 424 1235

UKAS HEFT