A molecular biology service is offered to aid in the diagnosis of lymphoproliferative disorders. PCR (Polymerase Chain Reaction) for IGH (Immunoglobulin heavy chain) and TCR (T cell receptor) clonality testing is performed using BIOMED-2 reagents.   

DNA can be extracted from formalin fixed paraffin embedded tissue, fresh tissue or blood.

Clonality tests available:

• IGH rearrangement
• TCRB gene rearrangement
• TCRG gene rearrangement

Sample requirements:

• Fresh tissue minimum 5mm³ for molecular studies (can be sent dry or in saline).

• Representative formalin fixed paraffin embedded tissue blocks.
• 5µm unstained paraffin sections.
• 5-10 ml peripheral blood in EDTA.
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Please see Labelling and completion of request form for guidence on specimen labelling.

The molecular biology service participates in the UK NEQAS Leucocyte immunophenotyping IGH/TCR clonally programme.